Seleˋˋo de leveduras da Regiˋo Amazˋnica para produˋˋo de protease extracelular

作者:Neves; Kilma Cristiane Silva; Porto; Ana Lucia Figueiredo; Teixeira; Maria Francisca Simas
来源:Acta Amazonica, 2006.
DOI:10.1590/S0044-59672006000300002

摘要

the objective of this work was to identify 50 yeasts isolates obtained from differents substracts from amazon region and to select one species of higher proteolytic activity. among the 26 identified species it had predominance of candida aquatica (12%) and candida tropicalis (10%). the submerged fermentation was carried out in malt extract supplemented with gelatin 1%, candida intermedia exhibited higher proteolytic activity, halo = 25 mm and 273u/ml, the maximum growth 30 oc, the cellular viability 6.2 x 106 ufc, in 48 hours, did not demonstrate pathogenicity characteristics and the best inoculum density tested was 3%, using 24 hours malt agar cultures.

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