科研之友
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摘要
objective: to determine the prevalence of rhinovirus infection in hospitalized young infants with acute bronchiolitis. methods: hospitalized children with acute bronchiolitis admitted to the hospital sˋo lucas/pucrs between may and september 2002 were selected prospectively. nasopharyngeal samples were assayed for respiratory syncytial virus, parainfluenza, influenza and adenovirus by immunofluorescence. for rhinovirus test a reverse transcription-polymerase chain reaction for picornavirus was used, followed by hybridization with rhinovirus specific probes. results: forty-five patients were selected for the study. the median age of the subjects studied was 2 months. positive samples for respiratory viruses were found in 35/45 (77.8%) subjects and 7/35 (20%) patients had dual infection. respiratory syncytial virus was detected in 33/35 (94%) cases. rhinovirus was detected in 6/35 patients (17%). conclusions: rhinovirus was the second most common agent detected in nasal secretions from young infants hospitalized with acute bronchiolitis.
