基于网络药理学的小陷胸汤治疗大肠癌的机制研究

作者：Gao Shi-Yong; Li Wan-Qiu; Wang Shuai; Shi Jia; Tan Hui-Xin^*

来源：Chinese Traditional and Herbal Drugs, 2022, 53(3): 773-782.

DOI：10.7501/j.issn.0253-2670.2022.03.017

• Cell cycle pathway
• Colorectal cancer
• DNA damage response pathway
• Gastrain signaling pathway
• Hepatitis B infection pathway
• Network pharmacology
• Xiaoxianxiong Decoction

Objective: To explore the mechanism of Xiaoxianxiong Decoction (小陷胸汤) on colorectal cancer by network pharmacology. Methods: The active components of Banxia (Pinelliae Rhizoma), Gualou (Trichosanthis Fructus) and Huanglian (Coptidis Rhizoma) in Xiaoxianxiong Decoction were collected and screened by TCMSP, and component-target network was constructed, GeneCards database was used to obtain colorectal cancer disease targets. Cytoscape software was used to identify the targets of Xiaoxianxiong Decoction in the treatment of colorectal cancer, and ClueGo was used to perform gene ontology (GO) and pathway enrichment analysis. Components and protein targets were docked by PyRx software to verify the accuracy. Results: Thirty-two active components of Xiaoxianxiong Decoction in the treatment of colorectal cancer, 181 corresponding protein targets and 9329 colorectal cancer disease targets were screened. By target fishing, 360 Hub targets of Xiaoxianxiong Decoction were obtained. GO enrichment analysis showed the biological process (BP) of Xiaoxianxiong Decoction in the treatment of colorectal cancer was related to NAD-dependent histone deacetylase activity, negative regulation of helioase activity, regulation of deoxyribonuclease activity, response to cholecystokinin, regulation of protein neddylation and so on. Molecular MF) was related to protein serine/threonine kinase activity, regulation of protein serine/threonine kinase activity, adenosine-triphosphate(ATP) binding, regulation of cysteine-type endopeptidase activity involved in apoptotic process and sequence-specific double-stranded DNA binding. Cellular component (CC) was related to spindle microtubule, histone deacetylase complex, nuclear nucleosome and ESC/E(Z) complex. The enrichment analysis of signal pathways showed that they were related with gastrain signaling pathway, cell cycle pathway, hepatitis B infection pathway and DNA damage response pathway. Molecular docking results showed that the active components screened from Xiaoxianxiong Decoction had good binding ability to the corresponding protein targets. Conclusion: Xiaoxianxiong Decoction can treat colorectal cancer by regulating gastrain signaling pathway, cell cycle pathway, hepatitis B infection pathway and DNA damage response pathway. ? 2022, Editorial Office of Chinese Traditional and Herbal Drugs. All right reserved.

• 单位
  哈尔滨医科大学; 哈尔滨商业大学