摘要

  A new lab-scale Internal Circulation (IC) anaerobic reactor fed by black liquid acid precipited wastewater was operated with increasing volumetric loading rate (VLR) under normal temperature.The reactor performance and the relationship between functional bacteria community structure and bioactivity in the anaerobic granular sludge were investigated by FISH and other conventional methods during the operation.Eventually, as the inlet COD was 8700mg/L,the COD removal rate was 49.46% ,the HRT was 37.38h,the VLR was 5.59 kgCOD/(n3 · d) and the SMA was 355.36 mLCH4/(d · g).Compared with the seed sludge, the abundance of the butyrate-oxidizing acetogenic bacteria Syntrophomonas spp.increased by 50.87%, the homoacetogenic bacteria Acetobaeterium species E.limosum increased by 214% , and methanomicrobium Methanomicrobiales increased by 26.39% , after 71 days operation.It was demonstrated that the anaerobic sludge granulation was significant in the new anaerobic reactor and such wastewater with high concentration and toxic like black liquid can be treated effectively by using the new anaerobic reactor.

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