Effect of Bushen Yisui Capsule(补肾益髓胶囊) on Oligodendrocyte Lineage Genes 1 and 2 in Mice with Experimental Autoimmune Encephalomyelitis

作者：杨涛; 郑琦; 赵晖; 张秋霞; 李明; 齐放; 李康宁; 房玲; 王蕾; 樊永平

来源：Chinese Journal of Integrative Medicine, 2016, 22(12): 932-940.

Objective: To study the effects of Bushen Yisui Capsule(补肾益髓胶囊, BSYSC) on the oligodendrocyte lineage genes(Olig) 1 and Olig2 in C57BL/6 mice with experimental autoimmune encephalomyelitis(EAE) in order to explore the remyelination effect of BSYSC. Methods: The mice were randomly divided into normal control(NC), EAE model(EAE-M), prednisone acetate(PA, 6 mg/kg), BSYSC high-dose(3.02 g/kg) and BSYSC low-dose(1.51 g/kg) groups. The mice were induced by immunization with myelin oligodendrocyte glycoprotein(MOG) 35-55. The neurological function scores were assessed once daily. The pathological changes in mice brains were observed with hematoxylin-eosin(HE) staining and transmission electron microscope(TEM). The protein expressions of myelin basic protein(MBP), Olig1 and Olig2 in brains were measured by immunohistochemistry. The m RNA expressions of Olig1 and Olig 2 was also determined by quantitative real-time polymerase chain reaction. Results: Compared with the EAE-M mice,(1) the neurological function scores were significantly decreased in BSYSC-treated mice on days 22 to 40(P<0.01);(2) the inflammatory cells and demyelination in brains were reduced in BSYSC-treated EAE mice;(3) the protein expression of MBP was markedly increased in BSYSC-treated groups on day 18 and 40 respectively(P<0.05 or P<0.01);(4) the protein expression of Olig1 was increased in BSYSC(3.02 g/kg)-treated EAE mice on day 40(P<0.01). Protein and m RNA expression of Olig2 was increased in BSYSC-treated EAE mice on day 18 and 40(P<0.01). Conclusion: The effects of BSYSC on reducing demyelination and promoting remyelination might be associated with the increase of Olig1 and Olig2.

• 单位
  中医学科学院; 首都医科大学