Efflux of potassium ion is an important reason of HL‐60 cells apoptosis induced by tachyplesin1

作者：Hai‐tao ZHANG; Jun WU; Hai‐feng ZHANG; Qi‐feng ZHU

来源：Acta Pharmacologica Sinica, 2006, 27(10): 1367-1374.

DOI：10.1111/j.1745-7254.2006.00377.x

• horseshoe crab
• tachyplesin
• intracellular potassium
• mitochondrial membrane potential
• HL-60 cell

Abstract Aim: To investigate the role of intercellular potassium in tachyplesin‐induced HL‐60 cells apoptosis. Methods: The concentration of intercellular potassium, cell volume and mitochondrial membrane potential were examined by flow cytometry. Results: The concentration of intercellular potassium reduced in a time‐dependent manner in tachyplesin‐treated HL‐60 cells. In addition, the loss of mitochondrial membrane potential was tightly coupled with the shrinkage of cell volume. Different caspase inhibitors protected against DNA degradation but did not prevent the loss of HL‐60 cell viability induced by tachyplesin. Ba2+, which was a kind of blocker of volume‐regulatory K+ channels, increased the viability of tachyplesin‐treated HL‐60 cells and maintained mitochondrial membrane potential and cell volume. Conclusion: Efflux of K+ was an important reason for apoptosis in tachyplesin‐treated HL‐60 cells. Efflux of K+ affected the viability of tachyplesin‐treated HL‐60 cells independent of the process of caspase activation.

• 单位
  广东医科大学