Role of Mineralocorticoid receptor (MR) and NEDD4‐2 in the regulation of ENaC and ROMK in the Distal Convoluted Tubule (DCT) and Cortical Collecting Duct (CCD)

作者：Zhang Dandan; Wu Peng; Duan XinPeng; Lin DaoHong; Staub Olivier; Ellison David H.; Wang WenHui

来源：The FASEB Journal, 2020, 34(S1): 1-1.

DOI：10.1096/fasebj.2020.34.s1.03827

Background ENaC and ROMK (Kir1.1) are expressed in the apical membrane of aldosterone‐sensitive distal nephron (ASDN). Aldosterone has been shown to play an important role in the regulation of ENaC and ROMK expression in ASDN. Also, NEDD4‐2 is an E3 ubiquitin ligase expressed in the ASDN and NEDD4‐2 has been shown to play a role in mediating the effect of aldosterone on ENaC. The aims of the present study are:1) To examine whether MR plays a similar role in regulating ENaC and ROMK in the DCT2 and CCD; 2) To examine whether NEDD4‐2 regulates ROMK channels in ASDN. Methods The whole‐cell recording technique has been used to measure the amiloride‐sensitive Na currents (ENaC) at −60 mV and TPNQ‐sensitive K currents (ROMK) at −40 mV in the DCT2/early connecting tubule (CNT) and in the CCD of tubule‐specific MR‐KO, NEDD4‐2 KO and corresponding WT mice on a normal rodent diet (0.4% Na and 0.9% K). Results Deletion of MR receptor decreased ENaC currents from10.2 pA/pf to 6.3 pA/pf (at −60 mV) in the DCT2/CNT but i...

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  Oregon Health & Science University; Oregon Health and Science University; new york medical college; New york medical college