A Novel Method Based on Ligase Detection Reaction for PGC-1汐 G646A Gene Polymorphism and its Genetic Effect on Meat Quality in Chicken

作者:Shu Jing Ting; Li Hui Fang; Zang Ying; Shan Yan Ju; Xu Wen Juan; Chen Kuan Wei
来源:Journal of Animal and Veterinary Advances, 2012.
DOI:10.3923/javaa.2011.2921.2925

摘要

The Peroxisome Proliferators-activated receptor-污 Coactivator-1汐 (PGC-1汐) was investigated as a candidate gene for meat quality and fatness traits in chicken because of its prominent role in muscle fiber specialization and adipogenesis. Variations of PGC-1汐+646G/A may associate with chicken meat quality traits. The objective of the current research was to investigate the association of this single nucleotide polymorphisms in PGC-1汐 gene with meat quality traits in Recessive White chickens and Qingyuan Partridge chickens. Genotyping was performed by Polymerase Chain Reaction-Ligation Detection Reaction (PCR-LDR) Method. Marker-trait association analysis indicated that there were significant associations between G646A genotypes and the trait of water loss rate. Researchers putatively drew the conclusion that this SNP in PGC-1汐 gene could be used as the potential molecular marker for meat quality trait in chicken.

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