Development of RNA Polymerase Ⅲ-Driven Reverse Genetics System for the Rescue of a Plant Rhabdovirus

作者：Xiaoyan Zhang; Kai Sun; Yan Liang; Chenglu Zhao; Zhenghe Li^*

来源：VIROLOGICA SINICA, 2021, 36(05): 1252-1255.

• net
• Development of RNA Polymerase
• Driven Reverse Genetics System for the Rescue of a Plant Rhabdovirus

<正>Dear Editor,De novo generation of negative-stranded RNA viruses(NSVs) requires efficient transcription of integral viral RNAs with precise termini from cloned plasmids. Studies during the past 25 years with animal NSVs have established the bacteriophage T7 RNA polymerase (Pol)-and endogenous Pol I-based transcription systems as the most efficient platforms for recovery of recombinant NSVs(Bridgen 2012). Unfortunately,

• 单位
  中国计量大学; 浙江大学; 水稻生物学国家重点实验室