科研之友
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摘要
Undernutrition in early life is associated with a number of acute and chronic sequelae, and recovering is a controversial issue. Even if undernutrition in Brazil is declining, studies have shown that about 31% of brazilian children still present severe or moderate malnutrition. The present study goal was to induce early malnutrition in rats and observe short- (undernourished) and long-term (after recovered) effects on defense cells involved in wound healing. Undernutrition was produced by separating the pups from the mother for 10 hours/day during the suckling period (21 days after birth). As controls were used rats at same age not submitted to suckling restriction. Undernutrition and recovering states were assessed by body weight. Skin wounds were made on the shaved backs of all, undernourished, recovered and their controls, under tribromoethanol anesthesia. Aninals were sacrificed 1, 3, 7 and 14 days after surgery and the tissues were properly prepared and observed under light microscopy. Our results showed that: 1) neutrophils, lymphocytes and macrophages couting in healing area were lower in undernourished and in recovered animals as compared with their controls; 2) there were a basal deficiency in lymphocytes and macrophages numbers in recovered animals but not in those acutelly undernourished. These results allow us to conclude that post-nattally undernourished animals submitted to a nutritional rescue time showed a complete recovery in physical weight, but in spite of the physical recovery, the wound healing showed less defense cell density in healing areas suggesting long-term sequelae of early undernutrition La desnutrici車n postnatal est芍 asociada a un conjunto de secuelas agudas y cr車nicas, y su recuperaci車n es a迆n asunto controvertido. En Brasil, a pesar de estar disminuyendo la desnutrici車n, algunos estudios han demostrado que alrededor del 31% de los ni os presentan malnutrici車n moderada o severa. El objetivo del presente estudio fue inducir una desnutrici車n precoz en ratas y observar los efectos inmediatos (desnutrici車n) y permanentes (depu谷s de la recuperaci車n) sobre las c谷lulas de defensa involucradas en la cicatrizaci車n. La desnutrici車n fue provocada separando las cr赤as de sus madres, por 10 horas diarias, durante el periodo de lactancia (21 d赤as de nacidos). Como contoles fueron usadas ratas de la misma edad no sometidas a restrici車n l芍ctea. Los estados de desnutrici車n y de recuperaci車n fueron evaluados por el peso corporal. Las heridas fueron realizadas en la piel rasurada del dorso de los animales desnutridos, recuperados y controles,
