科研之友
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摘要
tocopherols are well known constituents of vitamin e, and the main antioxidants in soybean. are natural antioxidants and stabilizers that can inhibit lipid degradation, reducing non-enzymatic oxidation of these compounds during storage of seeds, germination and initial development of seedlings. the objective of this work was to determine the level of 汐-tocopherol in four soybean seeds cultivars naturally and artificially aged. seeds of four soybean cultivars stored from 0 to 180 days in a dry chamber (natural aging) and subjected to high temperature and humidity (artificial aging) were analyzed for 汐-tocopherol content. the quantification of 汐-tocopherol in the soybean seeds was performed by high performance liquid chromatography (hplc) combined with the soxhlet extraction method. significant differences in 汐-tocopherol levels in seeds were observed for all cultivars and storage times. the 汐-tocopherol contents of the soybean seeds showed linear correlations with an increasing period of storage for all the cultivars studied. however, the artificially aged seeds had a higher content of 汐-tocopherol than those naturally aged.
