Estudo do polimorfismo gen谷tico da 汐 S1-case赤na em cabras, no Estado de Pernambuco, Brasil = Study of the genetic polymorphism of the 汐 S1-casein in goats of Pernambuco State, Brazil

作者：Ariosto Afonso da Silva; Manoel Adrio; George Chaves Jimenez; Madriano Christilis da Rocha Santos

来源：Acta Scientiarum - Animal Sciences, 2007.

• CSN1S1
• leite
• PCR-RFLP
• CSN1S1
• milk
• PCR-RFLP

O Estado de Pernambuco tem uma voca o pecu芍ria, especialmente, para aexplora o de caprinos. Dentre as prote赤nas, chamadas de case赤nas, a 汐S1-case赤na foi a primeira prote赤na comprovada com base no polimorfismo gen谷tico. Objetivando realizar a genotipagem de cabras criadas no sert o, agreste e zona da mata do Estado de Pernambuco, por meio da t谷cnica de PCR-RFLP, estudou-se o polimorfismo do gene da aS1-case赤na. Utilizaram-se 60 animais, divididos em tr那s grupos de 20 animais, das ra as Moxot車, Alpina Americana e SRD (Sem Ra a Definida). A extra o do DNA foi realizada com a utiliza o do protocolo fenol-clorof車rmio, e o gene da aS1-case赤na foi amplificado por meio da PCR (rea o da polimerase em cadeia). Em seguida, foi utilizada a enzima de restri o XmnI para obter a freq邦那ncia al谷lica das ra as estudadas. Encontrou-se, nos caprinos, os alelos da aS1-case赤na B e D que foram predominantes para a ra a nativa Moxot車 e animais SRD (100%), e os alelos C e D, para a ra a Alpina Americana(100%), concluindo-se que existem varia es gen谷ticas para o gene da aS1-case赤na do leite das ra as caprinas estudadas, embora se evidencie a proximidade gen谷tica entre a Moxot車 e SRD. The Pernambuco State, has been a livestock area, mainly for thecaprine exploration. Among the proteins, called caseins, the 汐S1-casein was the first proved protein with base in the genetic polymorphism. To genotype goats of the ※sert o§, ※agreste§ and ※zona da mata§ regions of the Brazilian State of Pernambuco, through the PCR-RFLP technique, we studied the polymorphism of the 汐S1-casein gene. Sixty animals were used, divided in three groups of twenty animals of the races Moxot車, American Alpine and UB (Undefined Breed). The DNA extraction was done by the phenol-chloroform protocol and the 汐S1-casein gene was amplified through the PCR (Polymerase Chain Reaction). Then, the restriction enzyme XmnI was used to obtain the allele frequency of the studied races. In the goats, we found the 汐S1-casein alleles B and D that predominantly were in the native raceMoxot車 and Ub animals (100%), the alleles, C and D for the American Alpine race (100%), concluding that there are genetic variations for the 汐S1-casein in the milk of the goat races studied in the region, although it was observed a genetic proximity between Moxot車 and UB.