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摘要
The effects of culture conditions, heavy metals and aromatic compounds on laccase production by the model strain of Phanerochaete chrysosporium were investigated, and degradation efficiency of the crude laccase to Congo Red was further detected. The results showed that the optimum medium for laccase production were glucose as carbon source and peptone as nitrogen source in the carbon-to-nitrogen ratio of 90. After 8d of culture, laccase activity was 911.1U/L. Mn2+ strictly controlled the laccase production by P. chrysosporium, while Cu2+ had little influence. When 4.0mmol/L Mn2+ was added, the laccase activity was 2 001.7U/L. The laccase production could be promoted by VA, 4-coumaric acid, vanillin aldehyde and vanillin acid, reaching the highest level of 1 459.1U/L, while coffee acid inhibited the laccase production slightly. 100U/L crude laccase could degrade 40mg/L Congo Red with degradation efficiency of 24.0%, and the degradation efficiency could increase to 87.7% by using VA as the redox mediator.
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