Physicochemical Characterization, in vitro Antioxidant and α-Glucosidase Inhibitory Activity of Polysaccharides from Rosa sterilis Fruit [刺梨多糖的理化性质、体外抗氧化和 α-葡萄糖苷酶抑制活性]

作者：Chen Q.; Li C.; Huang T.; Fu X.; Jia Q.

来源：Modern Food Science and Technology, 2019, 35(11): 114-119 and 253.

DOI：10.13982/j.mfst.1673-9078.2019.11.016

• Antioxidant
• Fractionation
• Hypoglycemic
• Polysaccharides
• Rosa sterilis fruit

Two main polysaccharide fractions (RSPs-40 and RSPs-60) were isolated from Rosa sterilis fruit by hot water extraction and fractional alcohol precipitation, respectively. The physicochemical characteristic, antioxidant and α-glucosidase inhibition activity of RSPs-40 and RSPs-60 were investigated. The results showed that the extraction yields of RSPs-40 and RSPs-60 were 2.62% and 1.81%, respectively. The total sugar contents of RSPs-40 and RSPs-60 were 42.5%±0.91% and 45.90%±0.37%, respectively. Protein contents were all 2.89%. The average molecular weights of RSPs-40 and RSPs-60 were 228.298 ku and 124.144 ku, respectively. Monosaccharide composition analysis showed that RSPs-40 mainly consisted of arabinose, galactose, glucose, fructose, and galacturonic acid in a molar ratio of 0.24:0.37:3.22:0.27:1.44, while RSPs-60 was mainly composed of arabinose, galactose, glucose, and galacturonic acid in a molar ratio of 1.58:2.06:2.37:1.69. The results of in vitro antioxidant assays showed that RSPs-40 and RSPs-60 exhibited good DPPH and ABTS free radical scavenging activity. The results of in vitro hypoglycemic assays showed that RSPs-40 and RSPs-60 exhibited stronger α-glucosidase inhibitory activity than acarbose. The antioxidant and α-glucosidase inhibitory activities of RSPs-60 were better than those of RSPs-40. Overall, these results suggested that the polysaccharides from R. sterilis fruit could be explored as a promising antioxidant and hypoglycemic dietary supplement.

• 单位
  华南理工大学