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摘要
Objective To investigate the prevalence of hepatitis D virus (HDV)infection among patients with chronic hepatitis B virus (HBV)infection in some regions of China. Methods Serum samples were collected from 3 131 patients with chronic HBV infection in 10 provinces, cities, and autonomous regions of China from March 2021 to June 2022, and anti - HDV IgG ELISA was used for the detection of all serum samples. Nested reverse transcription - polymerase chain reaction (nRT - PCR)was used to detect HDV RNA in anti - HDV IgG - positive samples, and the nRT - PCR amplification products of HDV RNA - positive samples were sequenced and analyzed to determine HDV genotype. The clinical features of anti - HDV IgG - positive patients were analyzed. The Mann - Whitney U rank sum test was used for comparison of continuous data between two groups, and the chi - square test or the Fisher's exact test was used for comparison of categorical data between two groups. Results The positive rate of anti - HDV IgG in the 3 131 patients with chronic HBV infection was 0. 70% (22/3 131), and that in the patients with chronic HBV infection in Inner Mongolia Autonomous Region, Xinjiang Uygur Autonomous Region, Beijing, and Hunan Province was 1. 81% (16/886), 0. 88% (2/226), 0. 28% (2/708), and 1. 00% (2/200), respectively;the patients with chronic HBV infection in Inner Mongolia Autonomous Region had a significantly higher positive rate of anti - HDV IgG than those in Beijing (P = 0. 004), and there was no significant difference between the other regions (P > 0. 05). Clinical features of the patients with chronic HBV infection in Inner Mongolia Autonomous Region showed that compared with the anti - HDV IgG - negative group, the anti - HDV IgG - positive group had a significantly higher proportion of patients with Mongol nationality (P = 0. 001), abnormal alanine aminotransferase (P = 0. 007), or antiviral treatment (P = 0. 029), as well as a significantly lower median HBV DNA level (P = 0. 030). A total of 19 HDV RNA - positive samples were identified, all of which had HDV genotype 1. Conclusion The prevalence rate of HDV varies greatly across different regions of China, with a higher prevalence rate of HDV in patients with chronic HBV infection from Inner Mongolia Autonomous Region. HDV genotype 1 is the predominant genotype in some provinces and cities of northern China. ? The Author(s) 2023.
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