科研之友
微信
新浪微博
Facebook
分享链接
摘要
background: galectin-3 (gal-3) is a b-galactoside-binding mammalian lectin. numerous studies have demonstrated that gal-3 plays an important role in tumor biology, acting in some events such as apoptosis, metastasis and malignant transformation. however, in carcinomas of head and neck, the real significance of gal-3 expression requires a better understanding. objectives: the aim of this paper was to evaluate gal-3 expression in tongue carcinomas experimentally induced in mice challenged with carcinogen 4-nitroquinoline-1-oxide (4nqo). material and method: forty-two c57bl/6 male mice were challenged with 4nqo in drinking water for 16 weeks and killed at different periods after induction. in each period, their tongues were removed, routinely processed, stained with hematoxylin and eosin (h%26e) and microscopically analyzed as to the presence of carcinoma. immunohistochemical test for gal-3 and a descriptive analysis of its expression in induced tumors were performed. results: by the end of the experiment, 15 tumors had been induced. in the non-neoplastic lingual epithelium, strong and weak immunoreactivity for gal-3 was noted in parabasal and superficial layers, respectively. in the basal layer, gal-3 expression varied from absent to weak. all the well-differentiated carcinomas showed weak immunoreactivity for gal-3, except in keratinized areas. the only case of poorly differentiated squamous cell carcinoma indicated strong immunoreactivity for gal-3. conclusion: our results show that malignant transformation is associated with reduced expression of gal-3, whereas its increased expression in poorly differentiated carcinoma seems to be connected with tumor aggressiveness.
