NIRII‐HDs: A Versatile Platform for Developing Activatable NIR‐II Fluorogenic Probes for Reliable In Vivo Analyte Sensing

作者：Qin Zuojia; Ren TianBing; Zhou Huijie; Zhang Xingxing; He Long; Li Zhe; Zhang XiaoBing; Yuan Lin

来源：Angewandte Chemie, 2022, 134(19).

DOI：10.1002/ANGE.202201541

• Fluorescent Probes
• Liver Injury
• Metastatic Tumors
• NIR-II Imaging
• In Vivo Sensing

Small‐molecule‐based second near‐infrared (NIR‐II) activatable fluorescent probes can potentially provide a high target‐to‐background ratio and deep tissue penetration. However, most of the reported NIR‐II activatable small‐molecule probes exhibit poor versatility owing to the lack of a general and stable optically tunable group. In this study, we designed NIRII‐HDs, a novel dye scaffold optimized for NIR‐II probe development. In particular, dye NIRII‐HD5 showed the best optical properties such as proper pKa value, excellent stability, and high NIR‐II brightness, which can be beneficial for in vivo imaging with high contrast. To demonstrate the applicability of the NIRII‐HD5 dye, we designed three target‐activatable NIR‐II probes for ROS, thiols, and enzymes. Using these novel probes, we not only realized reliable NIR‐II imaging of different diseases in mouse models but also evaluated the redox potential of liver tissue during a liver injury in vivo with high fidelity.(#br)A series of O‐HD‐like dye...

• 单位
  湖南大学; 化学生物传感与计量学国家重点实验室