重组酿酒酵母发酵生产瓦伦西亚烯及其衍生物

作者：Ouyang Xiaodan; Li Wen; Cha Yaping; Zhu Chaoyi; Li Shuang^*

来源：Food and Fermentation Industries, 2019, 45(20): 7-15.

DOI：10.13995/j.cnki.11-1802/ts.021558

• (+)-valencene
• cytochrome P450
• metabolic engineering
• Saccharomyces cerevisiae
• sesquiterpenoids

The metabolic pathway of Saccharomyces cerevisiae was modified to produce (+)-valencene and related sesquiterpenoids. The genes of cytochrome P450 monooxygenase HPO derived from Hyoscyamus muticus, valencene oxidase CnVO derived from Callitropsis nootkatensis and four of their mutants (HPOM, CnVO-3, CnVO-4 and CnVO-34) were introduced into S. cerevisiae. Their effect on (+)-valencene hydroxylation were investigated by co-expressed with cytochrome reductase AtCPR derived from Arabidopsis thaliana. Through resting cell assays, the catalytic efficiencies of strains expressing HPO and HPOM showed the best results, and HPOM was selected for future study. Strain S. cerevisiae PK2-24 was constructed by expressing (+)-valencene synthase CnVS from Callitropsis nootkatensis and overexpressing ADH1 (alcohol dehydrogenase) and tHMGl (truncated 3-hydroxy-3-methylglutaryl-coenzyme A reductase). After 158 h fermentation in a 3 Lbioreactor, total terpenoid yielded was 310.94 mg/L, which was 111 times higher than that of the original strain. In conclusion, this study presents a new candidate for industrial scale production of (+)-valencene and its related sesquiterpenoids. ? 2019 Authors.

• 单位
  华南理工大学