A Microarray Analysis of Angiogenesis Modulation Effect of Xuefu Zhuyu Decoction (血府逐瘀汤) on Endothelial Cells

作者：宋军; 陈文元; 吴立娅; 郑良朴; 林薇; 高冬; Ted J Kaptchuk; 陈可冀

来源：Chinese Journal of Integrative Medicine, 2012, (07): 502-506.

• Xuefu Zhuyu Decoction
• angiogenesis
• microarray
• Chinese herbs

<正>Objective:To study the angiogenesis modulation mechanism of Xuefu Zhuyu Decoction(血府逐瘀汤) on the endothelial cell line ECV304.Methods:ECV304 cells were treated with 2.5%Xuefu Zhuyu Decoctioncontaining serum(XFZYD-CS) for 24 h,48 h or 72 h.Thiazolyl blue tetrazolium bromide(MTT),fluorescence activating cell sorter(FACS),migration,adhesion and in vitro tube formation assays were conducted to confirm an angiogenesis effect of XFZYD at 3 time points.An analysis of angiogenesis regulator profiles was performed at 3 times with real-time polymerase chain reaction(RT-PCR) superarray.Results:At 48 h,XFZYD-CS induced ECV304 significantly improved cell viability,number in S phase,migration,adhesion and tube formation.At 24 h and 72 h, only cell migration was elevated.Microarray results showed that the expression of 27 angiogenesis-related genes was changed.Conclusion:XFZYD-CS treatment induced angiogenesis on ECV304 cells with significant cellcular changes occurring at 48 h and genetic changes as early as 24 h.

• 单位
  中医学科学院; Harvard Medical School